Figure 6
From: Herpes simplex virus UL56 interacts with and regulates the Nedd4-family ubiquitin ligase Itch
UL56 co-localizes with Ndfip1 and Ndfip2. (A) Schematic representation of UL56 and Ndfip proteins. HSV-2 UL56 (235 aa) contains three PY motifs and one predicted transmembrane domain (TMD). Ndfip1 (221 aa) and Ndfip2 (336 aa) contain two and three PY motifs respectively, and three TMD. (B-C) HEp-2 cells were transfected with plasmids encoding either Ndfip1-EGFP (pNdfip1-EGFP) (B) or Ndfip2-EGFP (pNdfip2-EGFP) (C) alone (left panels), or in combination with plasmids encoding UL56 (right panels). UL56 colocalized with Ndfip1-EGFP and Ndfip2-EGFP in co-expressing cells. (D-E) HEp-2 cells were transfected with pNdfip1-EGFP (D) or pNdfip2-EGFP (E), and subsequently infected with wild-type HSV-2. UL56 partially colocalized with Ndfip1-EGFP and Ndfip2-EGFP in infected cells. Scale bars, 10 μm.