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Figure 2 | Virology Journal

Figure 2

From: Analysis of adenovirus trans-complementation-mediated gene expression controlled by melanoma-specific TETP promoter in vitro

Figure 2

CD40L transgene expression by different recombinant Ad vectors. Serial 3-fold increasing amounts of AdTETP-IL-2/CD40L (A), AdCD40L/IL-2 (B), and AdCMV-CD40L (C) were used to transduce HeLa cervical carcinoma, SW480 colon carcinoma, and M000301 and M21L4 melanoma cells for 5 h. Cells were then washed, and CD40L was analyzed two days p.i. by flow cytometry. Results are shown as mean fluorescence intensity (MFI) of triplicate measurements. (D) Transduction of M000301 cells with AdCD40L/IL-2 without washing off virus. Analysis was performed as described for A-C.

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Virology Journal

ISSN: 1743-422X