Sustained viral load and late death in Rag2-/- mice after influenza A virus infection

Table 1 Continuous pulmonary damage and inflammatory response in influenza infected Rag2^-/^- mice

Group	Percentage of affected lung parenchyma	Severity of necrosis in affected bronchi	Necrosis of alveolar walls	Hyperplasia of type 2 AEC	Lymphocytic infiltrates around bronchi and vessels	Infiltrating neutrophils in airways and interstitium	Histiocytic infiltrates in alveolar walls and lumina
B6 PBS	<5%	0	0	0	0.2	0	0
B6 day2	6%	1.8	1.8	0	1.4	1.6	1.6
B6 day4	12%	4	3	0.2	2.8	1.8	2.4
B6 day6	50%	3.6	3.2	1	4.6	1.2	3.6
B6 day8	34%	2.6	2	1.8	4	0.4	3.2
B6 day10	24%	2	1	3.2	4.4	0.2	3.2
Rag2 PBS	<5%	0	0	0	0.2	0	0
Rag2 day2	<5%	1	1.6	0	1.4	1.2	0.6
Rag2 day4	18%	3.2	2.2	0.4	1.2	2.4	3.2
Rag2 day6	60%	4.2	2.8	1	0.6	3.6	4
Rag2 day8	18%	3.6	2.8	1.4	0.4	3.2	2.2
Rag2 day10	44%	3.4	3.2	3	0	4.4	3.6

Semi-quantitative scoring of pulmonary lesions and immune cell infiltrates (median value of each group, 3 or 4 mice per group, 5 successive sections per sample).
Lung tissue sections from female wild type or Rag2^-/-mice infected with 2 × 10³ FFU PR8 were prepared on the days p.i. indicated, stained with hematoxylin and eosin and evaluated for the indicated features by a semi-quantitative grading system with the scores of 0 to 5 representing the degree of severity as follows: 0 = no lesion; 1 = minimal; 2 = mild; 3 = moderate; 4 = severe; 5 = marked. Abbreviations: AEC, alveolar epithelial cell; B6, C57BL/6J mice; PBS, mice treated with Phosphate Buffered Saline as non-infected control; Rag2, Rag2^-/-mice.

ISSN: 1743-422X