Figure 1

Confocal imaging of MDCK cells over-expressing SH3 and NS1. pSH3 (SH3-expressing plasmid based on a modified pcDNA3 vector with the N-terminal Flag) and pNS1 (NS1-expressing plasmid with the pEGFP-c1 backbone) were transiently transfected into MDCK cells individually or together. 24 h post-transfection, cells were stained with anti-Flag antibody and Cy3-labeled secondary antibody. Detection of Cy3 channel seen as red (A, D, and G), GFP channel seen as Green (B, E, and H), and Merged these two channels (C, F, and I).