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Table 1 The optimal dilutions of the truncated gK and sera was determined by P/N*

From: Expressing gK gene of duck enteritis virus guided by bioinformatics and its applied prospect in diagnosis

Dilutions of duck anti-DEV positive antiserum and duck negative serum

The dilutions of the truncated gK (μg/ml)

 

2

3

4

5

6

7

8

10

1:10

1.803

1.648

1.853

1.932

2.119

1.921

1.901

1.938

1:20

1.962

1.776

2.036

2.143

2.072

2.230

2.174

2.108

1:40

2.038

1.937

2.253

2.203

2.436

2.455

2.389

2.551

1:80

1.991

2.078

2.573

2.578

2.236

2.658

2.510

2.618

1:160

1.886

2.040

2.613

2.660

2.581

2.737

2.885

2.542

1:320

1.676

2.116

2.910

2.543

2.720

2.781

2.878

2.767

1:640

1.581

2.149

3.046

2.201

2.680

2.699

2.690

2.440

1:1280

1.552

1.970

2.760

2.236

2.424

2.748

2.723

2.874

1:2560

1.491

1.808

2.537

1.981

1.949

2.418

2.524

2.542

1:5120

1.163

1.851

1.943

1.710

1.810

2.148

2.401

2.534

  1. *P/N represents OD450 (Duck anti-DEV positive serum)/OD450 (Duck negative serum); the dilutions of the truncated gK was 2, 3, 4, 5, 6, 7, 8 and 10 μg/ml, the result of the repeated test about the optimal dilution was same with the above.