Figure 6

Morphological analysis on HSV-1 capsids aggregating in the cell with CTMP-7 expression inhibited (X30, 000). KMB-17 cells transfected with pGE-1 or pGE-CTMP plasmid were infected at an MOI of 1 with HSV-1 and incubated at 37°C. At 48 hour post-infection, samples of cell were harvested, and the enveloping process of viral capsids was observed by electron microscopy. a. HSV-1 capsids were observed aggregating in the area closing to nuclear membrane (NM) of cells transfected by pGE-CTMP. b. HSV-1 capsids were localized separately in nuclear and cytoplasma of cells transfected by pGE-1 mock plasmid. c. HSV-1 capsids were counted in difference EM fields. n = 3 for all fields. Error bars represent the standard error of the mean.