Figure 4
From: Egress of HSV-1 capsid requires the interaction of VP26 and a cellular tetraspanin membrane protein
Inhibition of CTMP-7 in cells by RNAi delayed the proliferation of HSV-1. a. Expression of CTMP-7 in KMB-17 cells was inhibited by pGE-CTMP containing siRNA targeted specifically to CTMP-7 mRNA. Cells transfected by pGE-CTMP or pGE-1 were harvested after 48 h, and lysates were analyzed by Western blotting using antisera raised in mouse immunized with CTMP-7. 1: Cells transfected with pGE-1; 2: Cells transfected by pGE-CTMP. b. Inhibition of CTMP-7 expression by siRNA reduced HSV-1 proliferation in fibroblasts. Growth curves of HSV-1 in KMB-17 cells transfected with pGE-CTMP were produced and analyzed. KMB-17 cells transfected with pGE-1, pGE-CTMP plasmid were infected at an MOI of 1 with HSV-1, and incubated at 37°C. At the indicated times post-infection, samples of cell supernatant were removed and the viral titer was determined by microtitrating assay. n = 3 for all time points. Error bars represent the standard error of the mean.