Figure 2

DENV-3 phylogenetic tree based on the NS5 partial gene sequences. The three was constructed using the method of Neighbor-joining with 1000 bootstrap replications. The genotypes are labeled according to the scheme of Lanciotti in 1994 [14] and Amarilla in 2009 [15]. DENV-1, DENV-2 and DENV-4 were used as outgroup. Branch lengths are proportional to percentage of divergence. Tamura Nei (TrN+G) nucleotide substitution model was used with a gamma distribution (G) of 0.5121. Bootstrap support values are shown for key nodes only (values < 70% not shown). The strains isolated D3/BR/Santos/A. albopictus 13/1999 is marked with a filled square. The GenBank accession numbers, species, the country of origin, and year of isolation are shown.