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Table 2 Betanodavirus propagation in the medaka cell lines

From: Susceptibilities of medaka (Oryzias latipes) cell lines to a betanodavirus

Cell line

Inoculum a (TCID50)

Viral titer (TCID50/ml) b

  

Day 1

Day 3

Day 5

Day 7

OLHNI-1

103

4.4 × 104 ± 1.2 × 104

4.9 × 108 ± 7.0 × 107

1.8 × 109 ± 7.5 × 108

NT

OLHNI-2

103

4.0 × 104 ± 0

4.0 × 108 ± 1.7 × 108

1.4 × 109 ± 4.0 × 108

NT

OLHE-131

103

3.3 × 103 ± 7.5 × 102

4.4 × 107 ± 1.2 × 107

2.6 × 108 ± 6.0 × 107

NT

OLKaga-e1

103

4.0 × 103 ± 0

2.9 × 108 ± 3.5 × 107

3.2 × 108 ± 8.5 × 107

NT

OLHdrR-e3

103

4.8 × 103 ± 8.0 × 102

1.9 × 108 ± 1.0 × 107

2.9 × 108 ± 3.5 × 107

NT

OLCAB-e3

106

2.9 × 105 ± 3.5 × 104

2.1 × 107 ± 4.0 × 106

2.5 × 107 ± 1.5 × 107

3.6 × 107 ± 4.0 × 106

OLCAB-e21

106

5.1 × 104 ± 1.9 × 104

4.1 × 106 ± 1.6 × 106

3.7 × 106 ± 1.9 × 106

1.9 × 106 ± 1.3 × 106

OLCAB-e31

106

3.6 × 105 ± 4.0 × 104

5.6 × 106 ± 0

5.6 × 106 ± 0

7.8 × 106 ± 2.2 × 106

OLF-136

106

1.1 × 105 ± 6.9 × 104

4.8 × 106 ± 8.0 × 105

7.8 × 106 ± 2.2 × 106

2.1 × 107 ± 1.1 × 107

OLME-104

106

4.8 × 104 ± 8.0 × 103

4.8 × 104 ± 8.0 × 103

3.6 × 104 ± 4.0 × 103

3.6 × 104 ± 4.0 × 103

E-11

103

2.7 × 104 ± 1.3 × 104

1.4 × 108 ± 1.3 × 108

1.4 × 108 ± 4.0 × 107

NT

  1. a. Medaka cells and E-11 cells (1.0-1.5 × 105) were inoculated with RGNNV of the indicated titers.
  2. b. Viral titers in the culture supernatant were determined by the TCID50 assay using E-11 cells at 30°C.
  3. Each data represents the mean and standard deviation from two independent experiments.
  4. NT, Not tested.
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Virology Journal

ISSN: 1743-422X