Figure 3

Humoral and Cellular Immunity. (A and B) ELISA data show an increase in influenza specific IgM and IgG at 7 DPI compared to mock-infected control animals. These data show that there is a dose-dependent increase in antibody response. Bars indicate the average difference per group between log₂-transformed end-point dilutions from pre-infection serum and post-infection serum. (C) Neutralization titer analysis was performed in order to detect the presence of influenza-specific neutralizing antibodies in the serum. The presence of neutralizing antibodies was measured only on 7 DPI. No neutralizing antibodies were detected in the sera from control animals. Only 2 out of 4 animals from group 2 and 1 out of 4 animals from group 3 had detectable neutralizing antibodies, while all animals in groups 4, 5, and 6 had detectable neutralizing antibodies. (D, E, and F) The number of neutrophils and lymphocytes, as well as the total number of white blood cells, increased in animals infected with A/Brisbane/59/07 compared to mock infected control animals. Y axis indicates number of cells as 10³ cells per mm³.