Figure 4
From: The role of F1 ATP synthase beta subunit in WSSV infection in the shrimp, Litopenaeus vannamei
Coupling immunomagnetic separation on magnetic beads with western blot for detection of the interaction between BP53 and WSSV. Line marker, pre-stained protein molecular mass markers (MBI, USA); Line 1, SDA-PAGE of shrimp gill CMPs; Line 3, SDS-PAGE of the eluted components on dynabeads coated with WSSV particles after flowed with shrimp gill CMPs; Line 2 and 4, identification of BP53 using anti-rBP53 antibody by western blot. The samples loaded in Line 2 was shrimp gill membrane proteins, as same as Line 1; The samples loaded in Line 4 was the eluted components on dynabeads coated with WSSV particles after flowed with shrimp gill membrane proteins, as same as Line 3.