Table 1 Oligonucleotide primers used for nPCR amplification of WUPyV, KIPyV, MCPyV and simian polyomavirus LPyV
Primer | Virus region | Position | Amplification reaction | Product size (bp) | Annealing temperature (°C) | Sequence (5'-3') |
|---|---|---|---|---|---|---|
WU1 | WUPyV (VP1)a | 1730-1750 | First | 505 | 55 | CCCACAAGAGTGCAAAGCCTTC |
WU2 | 2234-2213 | AGGCACAGTACCATTGGTTTTA | ||||
WU3 | 2044-2063 | Nested | 164 | 50 | AGTTTTGGTGCTTCCTKTSC | |
WU4 | 2207-2188 | TACAGTATACTGAGCAGGC | ||||
KI1 | KIPyV (VP1)b | 1684-1704 | First | 378 | 59 | GCTGCTCAGGATGGGCGTGA |
KI2 | 2061-2043 | CAGKGTTCTAGGGTCTCCTGGT | ||||
KI3 | 1899-1918 | Nested | 190 | 54 | GTTGCTTGTTGTACCTCTAG | |
KI4 | 2088-2067 | AATTGTATAGGTAGTTGGGCCT | ||||
MC1 | MCPyV (TAg)c | 1716-1736 | First | 477 | 55 | GCCTGTGAATTAGGATGTATTT |
MC2 | 2210-2198 | CATTTCTGTCCTGGTCATTTCCA | ||||
MC3 | 2010-2033 | Nested | 183 | 50 | GCCCATTATCTAGACTTTGCAAA | |
MC4 | 2192-2173 | TCTAACCTCCTTTTGGCTA | ||||
MC1b | MCPyV (VP1)c | 3174-3194 | First | 440 | 58 | GGCTTTCTTTTTGAGAGGCCT |
MC2b | 3613-3592 | AGTGGGCCCTCTATGCAAAGGA | ||||
MC3b | 3276-3297 | Nested | 240 | 54 | TTGGGTAAACAGTTTTCTCCTG | |
MC4b | 3515-3493 | TGCCTAGATATTTTAATGTTACT | ||||
MC1c | MCPyV (VP1/2/3)c | 4228-4252 | First | 265 | 53 | GAATTAACTCCCATTCTTGGATTCA |
MC2c | 4492-4472 | TTGGCTTCTTCCTCTGGTACT | ||||
MC3c | 4264-4286 | Nested | 198 | 53 | ATTTGGGTAATGCTATCTTCTCC | |
MC4c | 4461-4439 | GGATATATTTCTCCTGAATTACA | ||||
LN1 | LPyV(VP2/VP3)d | 1542-1564 | First | 423 | 54 | GGCACACCAAAGAGTAACTCAAG |
LN2 | 1965-1943 | CAGGTCATGTCTTCATTTAGGAG | ||||
LN3 | 1617-1639 | Nested | 232 | 54 | GGAAGTGGAGCTTAATAAATTGG | |
LN4 | 1863-1849 | ATATCCATACAAGTCCTCAGAAG |