Figure 1

PCR amplification of DPV gE gene and identification of the recombination vector. A. Result of PCR amplification for DPV gE gene. Lane 1, the amplified product of DPV gE (about 1490bp); Lane 2, DNA marker 2000; B. Identification of the recombination vector pMD18/DPV-gE and pET32a/DPV-gE by restriction enzymes digestion. Lane 1, DNA marker 15000; Lane 2, the recombinant plasmid pET32a/DPV-gE was digested with EcoRI and XhoI (the PCR products with 1490 bp and the pET32a vector about 5,900bp) Lane 3, the recombinant plasmid pMD18/DPV-gE was digested with EcoRI and XhoI (the PCR products with 1490 bp and the pMD18-T vector about 2,700bp); Lane 4, DNA marker 2000.