Figure 1

Construction and characterization of Vpr plasmids for oligomerization studies in live cells using BiFC analysis. (A) Schematic representation of Vpr^wt fused with Venus-N terminal or Venus-C-terminal fragments. (B) Expression of Venus-C Vpr^wt and Venus-N Vpr^wt was assessed in HEK293T cells by transient transfection. HEK293T cells were transfected with Venus-N-Vpr^wt or Venus C-Vpr^wt expression plasmids or Vector control plasmid, and assessed by Western blot. (C) Subcellular localization pattern of Vpr^wt or Venus-Vpr^wt fusion proteins was assessed in HeLa cells by transient transfection. HeLa cells were transfected with Vpr^wt or Venus C - Vpr^wt or Venus N - Vpr^wt expression plasmids or Vector control plasmid, and assessed by Immunofluorescence for subcellular localization pattern using Vpr-specific antibody. Figure represents one of five independent experiments (n = 5) with similar results.