Figure 5From: Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a cloneHCV RNA levels in Huh-7.5 cell lines after full-length HCV-GFP RNA transfection determined by real-time RT-PCR. Stable Huh-7.5 cells were transfected with in vitro transcribed full-length HCV-GFP RNA. Cells were harvested after 0, 24, 48, 72 and 96 hours of post transfection. Total RNA was isolated and subjected real-time PCR. (black diamond) Huh-7.5 only, (black triangle) Huh-7.5 cells expressing NS3 antibody, (black circle) Huh-7.5 cells expressing control antibody.Back to article page