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Figure 3 | Virology Journal

Figure 3

From: Intracytoplasmic stable expression of IgG1 antibody targeting NS3 helicase inhibits replication of highly efficient hepatitis C Virus 2a clone

Figure 3

HCV positive- and negative-strand RNA detection by RPA showing the complete inhibition of HCV replication in stable Huh 7.5 cells expressing NS3 antibody (middle panel). Huh-7.5 cells were transfected with in vitro transcribed full-length HCV-GFP RNA. Cells were harvested after 0, 24, 48, 72 and 96 hours of post transfection. Total RNA was isolated and subjected to positive- and negative-strand HCV RNA detection and GAPDH mRNA by RPA. Upper panel: shows HCV positive- and negative-strand HCV RNA levels in Huh-7.5 cells transfected with full-length HCV-GFP mRNA. Middle panel: inhibition of HCV positive- and negative-strand RNA levels in Huh-7.5 cells expressing NS3 antibody. Lower panel: shows HCV positive- and negative-strand HCV RNA levels in Huh-7.5 cells expressing control antibody.

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Virology Journal

ISSN: 1743-422X