Pig no
|
Capture ELISAb
|
RT-PCRc
|
Virus isolation
|
IHCc
|
Abd
|
---|
4
|
-
|
-
|
-
|
-
|
-
|
5
|
-
|
+
|
+
|
ND
|
-
|
55
|
+ (lung, 4)
|
+
|
+
|
+
|
-
|
59
|
-
|
-
|
-
|
-
|
-
|
- a+ or -, samples positive or negative on capture ELISA, RT-PCR, virus isolation, IHC and antibody detection.
- bPositive identified tissue and the reciprocal titer of 10% tissue suspension in parentheses.
- cLung tissue samples were used in RT-PCR and IHC. Primer sequences and procedures were described in [10]. ND, not done.
- dBoth serum IgM and IgG detection assays were performed.