Characterizations of antibodies produced by hybridomas. (A) The 4-12% gradient gels were loaded with cell lysate equivalent to 2 μg of protein in each lane as follows, lane 1 and 2 (also lane 6 and 7) represent 2 preparations of NiV Malaysia infected Vero cell lysates; lane 3 and 8, NiV Bangladesh infected Vero cell lysate; lane 4 and 9, HeV infected Vero cell lysate; lane 5 and 10, control Vero cell lysate. After gel separation and transferring, membranes were probed with culture supernatant from 1A11 and 2B10. (B) 293T cells were transfected with NiV Malaysia P, V, W, C or N proteins expressed from plasmids. Ten μL of each cell lysate was separated by SDS-PAGE. Monoclonal antibodies purified from cloned hybridomas were diluted 1 to 2000 and incubated with transferred membranes. Lane M, ladder of MagicMark™ XP Western Protein Standard from Invitrogen.