Gel | Cy2(blue) | Cy3(green) | Cy5(red) |
|---|
1 | pool | A1 (Control1) | C2 (H168_2) |
2 | pool | C1 (H168_1) | E3 (N168_3) |
3 | pool | D2 (N96_2) | B2 (H96_2) |
4 | pool | B1 (H96_1) | E2 (N168_2) |
5 | pool | E1 (N168_1) | A1 (Control1) |
6 | pool | D3 (N96_3) | C3 (H168_3) |
7 | pool | B3 (H96_3) | A2 (Control2) |
8 | pool | A3 (Control3) | D1 (N96_1) |
- *Control and experimental samples (H96, H168, N96, N168) were labeled with either Cy3 or Cy5. Equal amounts of protein lysates from 3 uninfected negative control and 12 experimental samples were pooled as the internal standard, and labeled with Cy2. Each gel was loaded with 50 μg of Cy2-labeled protein pool, 50 μg of Cy3-labeled and 50 μg of Cy5-labeled samples as indicated. Three replicates were used by each experimental condition.
