Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis

Table 3 Different expression of proteins between H-PRRSV and N-PRRSV (N96/H96, H168/H168) inoculated lungs identified by MALDI-TOF or MALDI-TOF/TOF.

Master no.^a	Accession no.^b	Human protein (Abbr)	Average ratio^f	pValue^c	Mr (Da)	pI	Protein score^d	Sequence Coverage^e (%)
N96/H96
484	gi\|66352015	LMNA	-3.14	0.0075	65189	6.4	76	36
1173	gi\|50916342	HSPB1	-2.35	0.00063	14268	5.94	73	43
477	gi\|61867592	STIP1	-2.19	0.033	63056	6.02	41	1
604	gi\|410689	LAP3	-2.01	0.0092	55996	5.68	66	6
481	gi\|66352015	LMNA	-1.99	0.012	65189	6.4	89	30
374	gi\|27806351	EZR	-1.92	0.035	68832	6.06	70	1
460	gi\|66352015	LMNA	-1.83	0.035	65189	6.4	68	26
1303	gi\|40423533	AP3S2	-1.77	0.034	29516	11.16	100	37
1415	gi\|15082144	SOD1	-1.66	0.047	15408	6.04	78	21
1312	gi\|543113	TAGLN	-1.6	0.04	19326	6.96	61	11
520	Gi\|194034593	KIAA1468	-1.58	0.00093	120185	5.38	70	9
848	gi\|37800811	GPD1L	-1.55	0.029	22781	5.4	90	46
1292	gi\|182851479	VIM	1.6	0.011	18149	4.7	137	72
921	gi\|54020966	ANXA2	1.63	0.045	38795	6.49	52	7
942	gi\|148747594	RPLP0	1.63	0.026	34508	5.71	159	18
616	gi\|40426087	LAP3	1.74	0.0073	31073	5.64	193	14
1428	gi\|89886167	FABP5	1.82	0.018	15485	6.6	100	31
N168/H168
1235	gi\|959814	FUT1	-1.59	0.02	15873	9.52	184	35
1171	gi\|27806479	PKP1	1.7	0.008	81498	9.18	68	17
1519	gi\|6843240	HBA2	2.09	0.00028	13025	8.81	182	25
1506	gi\|6843240	HBA2	2.21	0.01	13025	8.81	220	30

a) Master no. is the unique sample spot protein number.
b) Accession is the MASCOT result of MALDI-TOF/TOF searched from the NCBI nr database.
c) The p value of ONE-ANOVA, p < 0.01, or Independent Student's t-test contrast, p < 0.05.
d) Protein score (based on combined MS and MS/MS spectra) and best ion score (based on MS/MS spectra) were from MALDI-TOF/TOF identification.
e) Sequence coverage (%) is the number of amino acids spanned by the assigned peptides divided by the sequence length.
f) Average ratios were calculated considering 6 replica gels and were calculated using Decyder software as the fold -change between normalized spot volume between N-PRRSV-infected lungs (N96 or N168) and H-PRRSV-infected lungs (H96 or H168) homogenates (Independent Student's t-test was based on the log of the ratio between N96 and H96, or between N168 and H168).

ISSN: 1743-422X