Proteome changes of lungs artificially infected with H-PRRSV and N-PRRSV by two-dimensional fluorescence difference gel electrophoresis

Table 2 Different expression of proteins between N-PRRSV (N96, N168) inoculated lungs and control identified by MALDI-TOF or MALDI-TOF/TOF.

Master no.^a	Accession no.^b	Human protein (Abbr.)	p Value^c	Mr(Da)	pI	Protein score^d	Sequence Coverage (%)^e
242	gi\|74008809	FLNA	0.0025	283130	5.74	123	1
316	gi\|5821963	ACO2	0.0088	83137	7.69	110	3
481	gi\|66352015	LMNA	0.0021	65189	6.4	89	30
612	gi\|194674843	NTF4	0.00044	33968	9.06	72	27
614	gi\|2624886	ALDH2	0.0045	54859	6.05	147	6
616	gi\|40426087	LAP3	0.0063	31073	5.64	193	14
638	gi\|47685624	ALDH9A1	0.0097	26192	5.43	125	16
656	gi\|190360675	FLOT1	0.0089	47554	7.66	74	27
666	gi\|126335980	CCDC13	0.0084	83367	7.68	69	19
938	gi\|194033965	ANXA1	0.0076	35689	7.16	75	29
1016	gi\|149409809	FECH	0.0033	49485	8.48	66	20
1038	gi\|87217590	DDAH2	0.0049	23244	5.33	88	39
1173	gi\|50916342	HSPB1	0.00092	14268	5.94	73	43
1200	gi\|544445	GSTP1	0.0036	23710	8.07	166	13
1250	gi\|17892411	PEBP1	0.0019	17055	5.74	149	22
1312	gi\|543113	TAGLN	0.0039	19326	6.96	61	11
1316	gi\|5031635	CFL1	0.0093	18719	8.22	43	6
1491	gi\|21545648	COX5A	0.0026	19379	6.88	82	14

a) Master no. is the unique sample spot protein number.
b) Accession is the MASCOT result of MALDI-TOF/TOF searched from the NCBI nr database.
c) The p value of ONE-ANOVA, p < 0.01, or Independent Student's t-test contrast, p < 0.05.
d) Protein score (based on combined MS and MS/MS spectra) and best ion score (based on MS/MS spectra) were from MALDI-TOF/TOF identification.
e) Sequence coverage (%) is the number of amino acids spanned by the assigned peptides divided by the sequence length.

ISSN: 1743-422X