Figure 2
From: The novel HSV-1 US5-1 RNA is transcribed off a domain encoding US 5, US 4, US 3, US 2 and α22
A RNA antisense to U S 3 accumulates in cells infected with wild-type virus. (A) Cultures of RSC in 25 cm2 flasks were mock infected (lanes 1 and 2) or infected with 10 PFU of HSV-1(F) (lanes 3 and 4) or R7802 (lanes 5 and 6) per cell. Total RNA was extracted 18 h later and reverse transcription was performed on 1 μg of total RNA. As a control, 1 μg of RNA was subjected to the same treatment, except that reverse transcriptase was omitted (lanes 1, 3, and 5). (B) Cultures of RSC in 25 cm2 flasks were either mock infected (lane 1) or infected with 10 PFU of HSV-1(F) (lane 2) or R7802 (lane 3) per cell. Total RNA was extracted 18 h later and aliquots of 10 μg of total RNA were separated on 1% formaldehyde agarose gel, transferred to Biodyne B nylon membrane and hybridized with single-stranded RNA probe 2. For the size determination molecular weight marker positions are shown. The amount of 18S ribosomal RNA is shown as the loading control.