Figure 5
Ultrastructure and virus-like particles (viral particles) in the cell of S. sclerotiorum hypovirulent strain XG36-1 observed under transmission electron microscopes (TEM). A, hyphal ultrastructure of hypovirulent strain XG36-1, the cytoplasm was granulated, the membranes of nuclei (N) and mitochondria (M) was disintegrated; only a few mitochondria existed, but the mitochondrial cristae was cavitated. B, hyphal ultrastructure of wildtype strain XG-13, the cytoplasm was well-distributed, plentiful mitochondria, and the membranes of nuclei (N) and mitochondria (M) was not destroyed. C, viral particles (white arrow) in cell of hypovirulent strain XG36-1, the size of individual particle is about 40 nm, several particles were enveloped by single-layer membrane. D, A few viral particles (red triangles) could be observed after negatively stained with 1% uranyl acetate on carbon-coated 400 mesh copper grids. Ultrastructure observation was carried out under FEI Tecnai G2 20 TWIN transmission electron microscope).