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Table 4 Sequences of the fourteen ribozyme templates used for PCR amplification

From: Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome

hRz #

Template sequence for amplification (5' to 3')

1

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAgttgcacCTGATGAGGCCGAAAGGCCGAAA cttttcctACAATTGTTTTTTTGAATTCATC

2

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAatcaggcCTGATGAGGCCGAAAGGCCGAAA cctaaaacACAATTGTTTTTTTGAATTCATC

3

CCGGTTCGAACCCGGGCACTACAAAAACCAACAActatgttCTGATGAGGCCGAAAGGCCGAAA ctgggctaACAATTGTTTTTTTGAATTCATC

4

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAtgataatCTGATGAGGCCGAAAGGCCGAAA ctcctatgACAATTGTTTTTTTGAATTCATC

5

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAgacacaCTGATGAGGCCGAAAGGCCGAAA cagtgaACAATTGTTTTTTTGAATTCATC

6

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAttcactgatCTGATGAGGCCGAAAGGCCGAAA cactatACAATTGTTTTTTTGAATTCATC

7

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAtggcctCTGATGAGGCCGAAAGGCCGAAA cttctttACAATTGTTTTTTTGAATTCATC

8

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAccacaggCTGATGAGGCCGAAAGGCCGAAA ccatgcACAATTGTTTTTTTGAATTCATC

9

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAgctgacatCTGATGAGGCCGAAAGGCCGAAA gttttgagtcACAATTGTTTTTTTGAATTCATC

10

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAaatggtctCTGATGAGGCCGAAAGGCCGAAA tttccatACAATTGTTTTTTTGAATTCATC

11

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAccatgcgCTGATGAGGCCGAAAGGCCGAAA cagcttccACAATTGTTTTTTTGAATTCATC

12

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAgagacCTGATGAGGCCGAAAGGCCGAAA cagcttcaACAATTGTTTTTTTGAATTCATC

13

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAttcatgCTGATGAGGCCGAAAGGCCGAAA tggcatACAATTGTTTTTTTGAATTCATC

14

CCGGTTCGAACCCGGGCACTACAAAAACCAACAAacctgttCTGATGAGGCCGAAAGGCCGAAA ttcaacagACAATTGTTTTTTTGAATTCATC

  1. Bold uppercase: conserved catalytic core. Lowercase: 5' and 3' arms of the hRz, respectively.
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Virology Journal

ISSN: 1743-422X