Table 2 Designations and base sequences of twelve primer sets evaluated to select optimal primer pairs for detection of the DENV-2 NGC RNA genome
Designation | Primer sequences (5'→3') |  |
|---|---|---|
Capsid1 | 2F: caatatgctgaaacgcgaga | 2R: ccatcactgttggaatcagc |
Capsid2 | 3F: caatatgctgaaacgcgaga | 3R: cgccatcactgttggaatc |
Capsid3 | 4F: gcgagaaatacgcctttcaa | 4R: ccatcactgttggaatcagc |
Capsid4 | 5F: tatgctgaaacgcgagagaa | 5R: cgccatcactgttggaatc |
Capsid5 | 6F: gcgagaaatacgcctttcaa | 6R: cgccatcactgttggaatc |
Capsid6 | 7F: atgctgaaacgcgagagaaac | 7R: ccctgctgttggtgggatt |
NS51 | 2F: tcaaaagcattcagcacctg | 2R: cacatttgggcgtaggactt |
NS52 | 3F: gcaatgtatgccgatgacac | 3R: caggtgctgaatgcttttga |
NS53 | 4F: gcaatgtatgccgatgacac | 4R: tcaggtgctgaatgcttttg |
NS54 | 5F: tggaggagccttagtgagga | 5R: acgtcccaaggttttgtcag |
NS55 | 6F: tgagcaagaaagagggagga | 6R: caggtgctgaatgcttttga |
NS56 | 7F: caaaagcattcagcacctgaca | 7R: gttaaagcgcttgcgaacct |