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Table 1 Tabulation of data for TCID50 and qRT-PCR analyses of hRz effectiveness

From: Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome

 

TCID50

qRT-PCR Cells

qRT-PCR Supernatant

 
 

Avg

SE

Avg

SE

Avg

SE

Avg % Red

Infected

4.39 × 106

7.12 × 105

1.77 × 106

4.17 × 105

2.92 × 106

1.38 × 106

0

Uninfected

2

1

3

2

3

1

N/A

No-hRz

1.88 × 106

5.94 × 105

1.37 × 106

2.53 × 105

1.60 × 106

6.46 × 105

41.71

Rz # 1

8.77 × 105

3.42106

1.62 × 106

3.13 × 105

9.46 × 105

2.70 × 105

52.03

Rz # 2

2.78 × 104

1.09 × 104

5.88 × 104

3.95 × 104

5.37 × 104

1.65 × 104

98.07

Rz # 3

1.16 × 106

6.89105

1.68 × 106

3.38, × 105

1.39 × 106

7.62 × 105

43.62

Rz # 4

2.37 × 106

1.14 × 106

1.99 × 106

2.61 × 105

1.51 × 106

7.58 × 105

27.39

Rz # 5

3.08 × 104

9.1 × 103

9.65 × 104

4.90 × 104

9.44 × 104

2.18 × 104

96.87

Rz # 6

2.33 × 105

1.16 × 105

1.65 × 106

1.97 × 105

6.45 × 105

1.76 × 105

59.74

Rz # 7

2.72 × 104

1.13 × 104

9.56 × 104

2.04 × 104

4.98 × 104

2.49 × 104

97.42

Rz # 8

1.87 × 105

1.26 × 105

6.46 × 105

2.51 × 105

4.95 × 105

2.43 × 105

80.76

Rz # 9

8.50 × 104

3.46 × 104

2.51 × 105

1.24 × 105

3.24 × 105

1.45 × 105

90.92

Rz # 10

4.07 × 105

2.34 × 105

5.11 × 105

1.39 × 105

1.36 × 105

4.71 × 104

85.74

Rz # 11

2.47 × 104

4.01 × 103

3.55 × 104

1.59 × 104

2.11 × 104

7.41 × 103

98.90

Rz # 12

5.49 × 105

1.61 × 105

5.51 × 105

3.17 × 105

3.43 × 105

2.06 × 105

81.54

Rz # 13

3.06 × 105

1.08 × 105

3.60 × 105

2.11 × 105

1.90 × 105

1.65 × 105

88.73

Rz # 14

1.98 × 105

1.22 × 105

2.63 × 105

1.07 × 105

2.63 × 105

2.20 × 105

90.53

  1. Averages of results from four separate infections (Avg) are presented for each type of analysis along with Standard Error of the Mean (SE). The average percent reduction for all tests (Avg % Red) relative to the Infected cell control is calculated in the final column.