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Figure 1 | Virology Journal

Figure 1

From: Effective suppression of Dengue fever virus in mosquito cell cultures using retroviral transduction of hammerhead ribozymes targeting the viral genome

Figure 1

A: Representative hRz structure and its DENV target sequence. hRz # 1 nucleotide sequence and structure is depicted. Nucleotides flanking the cleavage site (yellow box) in the envelope protein region of the DENV-2 target RNA are enlarged. The ribozyme cleaves the target RNA at the GUC triplet site following antisense recognition and base pairing of the two ribozyme arms. B: Nucleotide alignments of the Human (Hs), D. melanogaster (Dm), and Ae. aegypti (Aa) tRNAval. The position of the concensus internal A and B blocks of the RNA pol III promoter are indicated. C: Plasmid pLAeRzARH was derived from pLXRN as described in Materials and Methods. The RSV promoter was added to drive independent expression of the hygromycin resistance gene. Expression of each hRz is driven by the tRNAval internal RNA pol III promoter to optimize expression and translocation of the hRzs to the cytoplasm, and a stretch of 60As is attached to the 3' end of the hRz sequence for recruitment of RNA helicase.

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Virology Journal

ISSN: 1743-422X