Table 3 Oligonucleotide sequences of primers and probes used in AHV-1 FQ-PCR detection

Real-F^a

Forward

ttttcctcctcctcgctgagt

21

357–377

60

Real-P^a

Probe

ccctgggtacaagcgc

16

383–398

Real-R^a

Reverse

ggccgggtttgcagaagt

18

399–416

Con-F^b

Forward

ggacagcgtaccacagataa

20

246–265

498

Con-R^b

Reverse

acaaatcccaagcgtag

17

727–743

IC-F^c

Forward

acgagcgcaacccttga

17

1054–1070

92

IC-P^c

Probe

cggtttgtcaccggcagtcacct

23

1103–1125

IC-R^c

Reverse

acgtcatccccaccttact

19

1127–1145