Table 1 Intra- and inter-assay variability of the established FQ-PCR assay
From: Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1
Dilution of standard (copies/reaction) | Intra-assay | Inter-assay | ||||
|---|---|---|---|---|---|---|
Mean | SD | CV (%) | Mean | SD | CV (%) | |
1010 | 7.0 | 0.30 | 4.3 | 6.9 | 0.34 | 4.9 |
109 | 9.9 | 0.19 | 1.8 | 10.1 | 0.23 | 2.3 |
108 | 13.4 | 0.25 | 1.9 | 13.6 | 0.34 | 2.5 |
107 | 16.9 | 0.29 | 1.7 | 17.0 | 0.36 | 2.1 |
106 | 20.8 | 0.33 | 1.6 | 20.9 | 0.40 | 1.9 |
105 | 24.7 | 0.37 | 1.5 | 24.7 | 0.49 | 2.0 |
104 | 28.6 | 0.49 | 1.7 | 28.7 | 0.77 | 2.7 |
103 | 32.3 | 0.52 | 1.6 | 32.2 | 0.90 | 2.8 |
102 | 35.4 | 0.81 | 2.3 | 35.3 | 1.16 | 3.3 |
101 | 37.2 | 2.31 | 6.2 | 37.5 | 2.51 | 6.7 |