Skip to main content

Advertisement

Table 1 Intra- and inter-assay variability of the established FQ-PCR assay

From: Development of TaqMan® MGB fluorescent real-time PCR assay for the detection of anatid herpesvirus 1

Dilution of standard (copies/reaction) Intra-assay    Inter-assay   
  Mean SD CV (%) Mean SD CV (%)
1010 7.0 0.30 4.3 6.9 0.34 4.9
109 9.9 0.19 1.8 10.1 0.23 2.3
108 13.4 0.25 1.9 13.6 0.34 2.5
107 16.9 0.29 1.7 17.0 0.36 2.1
106 20.8 0.33 1.6 20.9 0.40 1.9
105 24.7 0.37 1.5 24.7 0.49 2.0
104 28.6 0.49 1.7 28.7 0.77 2.7
103 32.3 0.52 1.6 32.2 0.90 2.8
102 35.4 0.81 2.3 35.3 1.16 3.3
101 37.2 2.31 6.2 37.5 2.51 6.7