Evolution of the M gene of the influenza A virus in different host species: large-scale sequence analysis

Table 3 Sites under positive selection for human influenza

Gene	Domain^a	Position	dN/dS^b	eSLAC	iSLAC	tSLAC	eFEL	iFEL
M1		219	inf^c	0.0048	0.22	0.022	0.0032	0.013
M2	Ex	11	inf	0.074	0.23	0.33	0.017	0.0055
	Ex	12	inf	0.0068	0.32	0.022	0.0026	0.024
	Ex	13	inf	0.020	0.55	0.036	0.0025	0.064
	Ex	14	inf	0.069	0.37	0.19	0.019	0.025
	Ex	16	6.18	0.015	0.027	0.27	0.021	0.0017
	Ex	21	inf	0.071	0.37	0.19	0.036	0.040
	Ex	23	inf	0.052	0.27	0.19	0.037	0.024
	Tm	27	4.42	0.054	0.59	0.039	0.082	0.20
	Cy	57	3.62	0.21	0.16	0.57	0.050	0.024
	Cy	89	inf	0.002	0.12	0.016	0.0023	0.0046

The significance of SLAC and FEL results for positive selection levels are given as P-values.
^aEx indicates extracellular domain; Tr, transmembrane domain; and Cy, cytoplasmic domain.
^bdN/dS was calculated by eFEL.
^c"inf" means infinity as denominator is 0.
Bold values are those deemed to show significantly positive selection (P < 0.05).

ISSN: 1743-422X