Figure 5

Viral neutralization by anti E2 peptide goat antibodies. Purified mono-specific polyclonal antibodies against p36–p38 epitopes were used at 300 to < 12.5 μg/tube to bind HCV from infected sera and the immune-capture activity of each Ab was assessed by RT-nested PCR amplification. The 174 bp amplicon denotes the presence of captured virus. The immune-capture experiment was repeated 3 times with different serum samples. Each Ab displayed the same immune-capture activity with different infected sera. Negative control and binding specificity were assessed by replacing the anti peptide Ab by PBS and anti HBV Ab respectively.