Figure 2

Biochemical PrP^res typing in diagnostic target sites of aged cattle with BSE. PrP^res was analyzed at least five times on different gels with a core-binding antibody (mAb 6H4) by Western blot. a) Average molecular masses of the unglycosylated (red open circles), the monoglycosylated (green filled circles) and the diglycosylated band (blue rectangles) of PrP^res are depicted with the related standard errors of the mean (S.E.M.). The cut-off value for the molecular mass of the unglycosylated band to discriminate H-type from C-type BSE was defined as the average molecular mass of all samples under investigation (solid line) +/- 5% (dashed line). b) Tri-plot graph presenting the relative intensities of the un-, mono- and diglycosylated PrP^res moieties. The L-type BSE control (green square) is the sole sample that fell below the decision limit of 55% relative intensity of the diglycosylated PrP^res (blue dashed line). The H-type BSE control is indicated by a red triangle and the zebu Charly-04 by a yellow circle. Samples were derived from medulla oblongata at the level of obex but where this was not available hippocampus was examined (marked by asterisks). CS, clinical suspect; ES, emergency slaughter; FS, fallen stock; RS, routine slaughter.