Figure 7
Co-immunoprecipitation by an anti-flag mAb. Coomassie blue staining of a 18% SDS-PAGE. Bacterial lysates expressing flagged NTAIL, flagged IRF-3 RD, or histidine-tagged NTAIL were used. Purified unflagged XD was also used. Fl-t: flow-through (unretained fraction). R: retained fraction. Arrowheads show the light and heavy chains of the mAb, which are visible on the gel at around 25 and 55 kDa, respectively. Numbers 1 to 3 highlight IRF-3 RD, NTAIL and XD bands, respectively.