Figure 6
Analysis of N TAIL structural transitions in the presence of IRF-3 RD by EPR spectroscopy. Normalized room temperature EPR spectra of three spin-labeled NTAIL proteins (20 μM) either in the absence or presence of a four-fold molar excess of IRF-3 RD (left panel) or in the absence or presence of a two-fold molar excess of XD (right panel). Spectra were recorded in buffer D. The schematic representation of each spin-labeled NTAIL protein is shown. The spin-label is highlighted.