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Figure 4 | Virology Journal

Figure 4

From: Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection

Figure 4

Detection of episomal cDNA and isolation of cotton rat-HIV-1 chimeric DNA sequence from HIV-1 infected cotton rat cells. (A), CCRT K4 cotton rat clone infected with HIV-1 MN and incubated for 4 days before isolation of genomic DNA for the detection of episomal cDNA. Numbers on the top represent the amounts of DNA (ng) used as input for each PCR reaction. HIV and β-actin panels indicate the fragments amplified in each panel. (B), DNA preparations from infected (HIV) and uninfected (mock) CCRT K4 cells were subjected to PCR amplification using one primer targeting a genomic cotton rat sequence and another primer targeting the sequence of HIV-1 MN LTR. The indicated band (arrow) was isolated, cloned and its sequence (GenBank AY703985) confirmed the integration of HIV-1 into the cotton rat genome.

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Virology Journal

ISSN: 1743-422X