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Figure 1 | Virology Journal

Figure 1

From: Expression of Human CD4 and chemokine receptors in cotton rat cells confers permissiveness for productive HIV infection

Figure 1

Isolation and cloning of cotton rat cells expressing human co-receptors for HIV-1. (A), VCRT C17; (B), CCRT C4; (C), CCRT C5; (D), CCRT C6; and (E), CCRT K4, were established which expressed human CD4 molecule, with human CXCR4 or CCR5, as indicated. The expression of these surface molecules was measured by FACs using fluorescent antibodies against the indicated molecules (CD4, clone RPA-T4; CXCR4, clone 12G5; CCR5, clone 2D7) (filled profile) and compared to the staining obtained of the same cells with control antibody isotypes (empty profiles). (F) Staining profile of parental CCRT cells with the above mentioned antibodies, as indicated.

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