FACS analyses of isolated DCs. Subconfluent monolayers of DCs isolated from STAT-/- and parental STAT+/+ 129SVE mice grown in GM-CSF containing media were infected with 10 PFU/cell of McKrae. At the indicated times post infection the cells were harvested and reacted with Annexin-V or 7-ADD dye to analyze apoptosis and cell death respectively and FACS analysis was performed (see Materials and Methods). Since DCs isolated from STAT-/- mice did not survive to 48 h post infection, FACS was done at 12 and 24 hr post infection for STAT1-/- parental STAT+/+ 129SVE DCs. The percent of cells positive are shown. The results are the average of two experiments.