Figure 2

The RNA-binding properties of unfused TGBp2. A. Effect of salt concentration on the RNA-binding activity of unfused TGBp2. After UV-crosslinking with TGBp2, the ³²P-labeled viral RNA was digested with RNase ONE. The protein sample was then run on a Tricine SDS-polyacrylamide gel before autoradiography. B. Non-specific RNA binding of TGBp2. The BaMV RNA (220 nucleotides), sigA RNA (400 nucleotides), and flgM RNA (164 nucleotides) were synthesized using the linearized pBaHB, psigA-100-2.4 and pEd21d-flgM plasmids as templates, respectively. The RNA-binding assay was carried out using the same method as described in A. Both autoradiography (upper panel) and Coomassie blue staining (bottom panel) of TGBp2 are shown for each panel.