Figure 3

Analysis of ODN-mediated reduction of HSV-1 titer in Vero cells and primary lung fibroblasts. A, B and C. Transfection of ODNs. Vero cells (A, C) and primary lung fibroblasts (B, MRC-5 cells) were treated with 50 nM of the indicated ODNs or PBS for 5 to 16 h in the presence of transfection reagent. The medium was changed to DMEM and cells were incubated for 1 h. The cells were infected and after 24 h the HSV-1 DNA levels in the supernatants were determined by quantitative PCR. The mean value of all experiments +SE is shown. The total number of infections performed (n) is indicated below the graph. **P ≤ 0.01, *P ≤ 0.05 against PBS. C. Comparison of ODN 5 and siRNA UL5.2 targeting the same region of UL5 mRNA, performed as described in A. As negative control the commercially available siRNA siGLO RISC-Free (Dharmacon) was used.