Figure 1

Model for ODN-mediated inhibition of HSV-1 and ODNs used. A. The HSV expression cascade during replication consists of the expression of (1) immediate early genes (IE) and (2) early (E) genes from the circularized HSV genome, of (3) leaky late genes (LL) from the parental genome and progeny genomes and of (4) late genes (L) from progeny genomes. ODNs target mRNAs of early and leaky late gene products, a component of the helicase/primase complex, the single strand binding protein (SBB), the polymerase as well as the tegument protein VP16, which is a component of the virion and facilitates IE gene expression in the next round of infection. B. Target sites (bold) and sequences of the oligonucleotides used. C. ODN A, S2, Sc, Cont and asS2 served as negative controls.