Sensitivity of the HA-mediated transduction to lysosomotropic agents. The 293T target cells were first treated with either ammonium chloride (NH4Cl) or Bafilomycin A1 (BAF) at various concentrations prior to the challenge by the HA(QH)/HIV, Ebola GP/HIV, and HIVgp120/41/HIV (labeled as GP120/HIV here) pseudovirions. The luciferase activities of the infected cells were determined 48 h post-infection. The data of the NH4Cl treatment at the concentration of 3.125 mM and the Bafilomycin treatment at the concentration of 3.125 nM are shown here. Values are means of triplicate samples ± SD.