DENV-binding proteins from Ae aegypti salivary glands detected with anti-E monoclonal antibodies. Total proteins from Ae aegypti salivary gland extracts were treated as described in Figure 1. After transfer, membrane sheets were incubated with either: DEN1 or DEN4 reference strains. Virus binding was then detected using anti-E Mabs. The estimated size of the DENV-binding proteins are indicated in kilodaltons (kDa) on the right side of the figure.