DENV-binding proteins from Ae aegypti salivary glands detected with anti-DENV polyclonal antibodies. Total proteins from Ae aegypti salivary gland extracts were separated on a SDS-PAGE (Electr) and transferred onto a nitrocellulose membrane. Membrane sheets were then incubated with either: DENV reference strains (DEN1 to DEN4); a semi-purified non-inoculated suckling mouse brain extract (NEG); or a DEN4 clinical isolate. Virus binding was detected using anti-DENV HMAF. Migration of the molecular weight markers and the estimated size of the DENV-binding proteins are indicated in kilodaltons (kDa), respectively on the left and on the right side of the figure.