Figure 4
An in vitro binding assay of LDAg peptides to CHC (1–107) fragment produced from E. coli. (A) Expression profile and purification of the fragment of CHC in E. coli. The top gel was stained with Coomassie blue and the bottom gel was detected by anti-His antibody. The protein makers are indicated to the left of the gel. (B) E. coli expressed GST and GST fusion proteins as shown in Fig. 3 were incubated with purified CHC as shown in (A). The GST pull-down products were analyzed by SDS-PAGE and silver stained. The proteins used for the pull-down are indicated above the gel and their migration positions are indicated by a black arrowhead (GST-LD), a gray arrowhead (GST) and a white arrowhead (CHC). Open circle (lane 1) indicates an unknown protein co-purified with CHC from E. coli.