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Figure 4 | Virology Journal

Figure 4

From: Production of recombinant AAV vectors encoding insulin-like growth factor I is enhanced by interaction among AAV rep regulatory sequences

Figure 4

Western blot analysis of Rep protein expression by 293 cells co-transfected with pAAV-IGF-I, the adenovirus helper plasmid pHelper and the designated AAV helper plasmids. Plasmid pAAV-RC (lane 1), pAAV-RC/ΔATG (lane 2), pAAC-RC/ACG (lane 3), pAAV-RC/p19/ACG (lane 4), pAAV-RC/p5 (lane 5) and pAAV-RC/p5/ACG (lane 6). As a control, cells were co-transfected with the adenovirus helper plasmid pHelper and pAV1 (lane 7). Two days after the co-transfection cells were harvested, lysates were separated by SDS-PAGE and Western blotting was performed as described in Materials and Methods.

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Virology Journal

ISSN: 1743-422X