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Figure 2 | Virology Journal

Figure 2

From: Epstein-Barr Nuclear Antigen 1 modulates replication of oriP-plasmids by impeding replication and transcription fork migration through the family of repeats

Figure 2

Two models to explain decreases in copy number when oriP plasmids contain an FR with 20 or more EBNA1 binding sites. DS is represented as a striped oval, and EBNA1-binding sites in FR are represented as black filled circles. For simplicity, only ten binding sites are shown. EBNA1 dimers bound to DS or FR are represented as gray ovals. (A) Replication factor titration model. EBNA1 bound to FR is proposed to non-functionally titrate cellular replication factors, such as ORC proteins, away from EBNA1 bound to DS, thus decreasing replication initiation events at DS. The titration efficiency is proportional to the amount of EBNA1 at FR, which in turn is dependent on the number of EBNA1 binding sites in FR. (B) The replication fork barrier model in which EBNA1 bound to FR is proposed to act post-initiation to impede the progression of replication forks initiated at DS. A decreased efficiency of progression is indicated by the gradation in line color from black to light gray. The strength of this barrier is proportional to the amount of EBNA1 present at FR, which is also dependent on the number of EBNA1 binding sites in FR.

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Virology Journal

ISSN: 1743-422X