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Figure 4 | Virology Journal

Figure 4

From: Engineering the vaccinia virus L1 protein for increased neutralizing antibody response after DNA immunization

Figure 4

Expression of truncated L1 proteins detected by Western blotting. BS-C-1 cells were transfected with plasmids and cells (A) and media (B) were harvested separately and analyzed by SDS-PAGE followed by Western blotting with polyclonal L1 antibody. In panel C, the Western blot of the cell lysate was probed with antibody to glyceraldehyde 3-phosphate dehydrogenase as a loading control. Proteins were detected by chemiluminescence. Lanes: 1, pL1op; 2, psL1op; 3, pL1optr; 4, psL1optr; 5, empty vector; 6, lysate from VACV-infected cells. The positions and masses in kDa of marker proteins are shown on the left.

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Virology Journal

ISSN: 1743-422X