Figure 3

Optimization of conditions for platelet Ad binding. Platelets were isolated as above and incubated with Ad (MOI = 10 (a) or MOI = 100 (b), 1 hr, RT, 1:1 antibody dilution). Direct flow cytometry was used to measure Ad binding as FITC-positive platelet events. (c,d) Optimization of the FITC-labeled anti-Ad hexon antibody dilution (c), 1:1 (d) 1:8 dilution, RT, 1-hr]. 1:2 and 1:4 antibody dilutions resulted in levels of Ad binding detection between 1:1 and 1:8 (not shown). Figures representative of n = 4.