Disorder predictions of potyviral VPgs. Five predictors were used: PONDR® VLXT, FoldIndex©, DISOPRED2, VSL2, IUPred. The location of predicted disordered (in the order provided by the above-listed predictors) was schematically represented by lines along the VPg sequence. Numbering indicates the VPg length. Highly conserved regions (grey) and consensus predicted α-helices and β-strands are indicated. The conserved tyrosine (Y) involved in VPg urydylylation and the sites (*) involved in virulence are indicated. The VPgs experimentally demonstrated to be disordered are shaded. LMV Lettuce mosaic virus, PVY Potato virus Y, PVA Potato virus A, TEV Tobacco etch virus, TuMV Turnip mosaic virus, BYMV Bean yellow mosaic virus.