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Figure 1 | Virology Journal

Figure 1

From: Increased production of viral proteins by a 3'-LTR-deleted infectious clone of human T-cell leukemia virus type 1

Figure 1

Increased expression of viral antigens in 293T cells transfected with an HTLV-1 clone with a deleted 3' LTR. (A) Molecular clones of HTLV-1 constructed by overlapping PCR. The complete genome, pFL-MT2 (upper), and a 3'-LTR-deleted clone in which the 3' LTR was replaced with that of the endogenous human gene (lower). (B) Increased expression of the p19 gag protein was detected by ELISA after transfection with the 3'-LTR-deleted clone. The amount of p19 gag in the concentrated (200-fold) medium from cells transfected with the complete molecular clone, pFL-MT2, pFL-MT2 + SV40 ori, Δ3' LTR, or Δ3' LTR + SV40 ori. (C) RT-PCR detection of the doubly spliced tax mRNA in cells transfected with the complete molecular clone or with the Δ3' LTR clone 2-3 days after transfection or with pUC19 two days after transfection. MT-2, total RNA extracted from the HTLV-1-infected cell line MT-2 was used as the positive control; N, total RNA extracted from 293T cells was used as the negative control.

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Virology Journal

ISSN: 1743-422X